An Early Molecular Biomarker Study in Pre-eclampsia

Preeclampsia (PE), a hypertension condition associated with pregnancy that manifests after 20 weeks of pregnancy,may be brought on by faulty placental development and poses serious risk to both the mother fetus. The disease can bedetected only once symptoms arise, there is need for biomarker, which makes early detection possible, andproper management done at an stage pregnancy. Even though many studies were globallyregarding role microRNAs as molecular biomarkers in pre-eclampsia, no literature showed effect microRNA inpreeclampsia Indian population. aim study was find marker detect atan stage, even before arise. Placental MicroRNAs studied using Next-generation Sequencing 60Preeclamptic groups ( 30 Early Onset,30Late onset preeclampsia), control selected; miRNA profiling wasdone Illumina sequencing, quantifier did downstream analysis identification, quantification, expression profiling.pl, script, miRDeep2.pl, script. Total RNAs extracted from tissues cells TRIzol reagent purified. Therelative miR-483-5p or determined: micro-RNA 483-5p expressed significant quantity inEarly Onset preeclamptic samples compared Late normal samples. miR analyzed, genetargets found computational methods. From this study, it used earlybiomarker identification preeclampsia.